Quick Overview for EGF 蛋白 (ABIN934920)
抗原
See all EGF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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序列
- MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
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产品特性
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Purified recombinant Human EGF protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 0.89 ng/mL . Measured in a cell proliferation assay using mouse Balb3T3 cell.
Molecular weight on SDS-PAGE will appear higher.
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Epidermal Growth Factor (EGF) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
- EGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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实验流程
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Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/mL - 1 µg/mL Detection method: BrdU assay
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in PBS, pH 7.4.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
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Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: Epidermal growth factor protein, Urogastrone protein, Epidermal growth factor protein, epidermal growth factor (beta-urogastrone), EGF protein, Pro-epidermal growth factor protein -
分子量
- 6.3 kDa (54 AA)
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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