MAP2K4 蛋白
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北京 101111
Quick Overview for MAP2K4 蛋白 (ABIN7939330)
抗原
See all MAP2K4 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant MAP2K4 (MEK4) protein
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Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-399) protein (GST tag)
MAP2K4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (GST tag,His tag)MAP2K4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-144) protein (His-IF2DI Tag)MAP2K4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (Myc-DYKDDDDK Tag)MAP2K4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 2-397) protein (His-SUMO Tag)MAP2K4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Recombinant MAP2K4 (MEK4) protein in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))
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别名
- MAP2K4
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背景
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Protein Background: MAP2K4 (Dual specificity mitogen-activated protein kinase kinase 4), also known as JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1 and SKK1. MAP2K4 is a dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Short Description: Recombinant MAP2K4 (MEK4) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_003001.1) with an N-terminal FLAG tag. The molecular weight of the protein is 45.58 kDa.
Aliases: JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SEKR1, SKK1
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途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
抗原
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