MAP2K4 Protein (His-GST)
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Quick Overview for MAP2K4 Protein (His-GST) (ABIN7320204)
抗原
See all MAP2K4 蛋白蛋白类型
宿主
资源
纯度
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标记
- This MAP2K4 protein is labelled with His-GST.
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原理
- Recombinant Mouse MEK4/MAP2K4 Protein (His & GST Tag)
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序列
- Ala 2-Asp 397
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产品特性
- A DNA sequence encoding the mouse MAP2K4 (P47809) (Ala 2-Asp 397) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-399) protein (GST tag)
MAP2K4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-144) protein (His-IF2DI Tag)MAP2K4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (Myc-DYKDDDDK Tag)MAP2K4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 2-397) protein (His-SUMO Tag)MAP2K4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))
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别名
- MEK4/MAP2K4
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背景
- JNKK1,MEK4,MKK4,PRKMK4,Sek1,Serk1,Dual specificity mitogen-activated protein kinase kinase 4, also known as MAP kinase kinase 4, MAPKK4, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, JNKK and MAP2K4, is a protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MAP2K4 / JNKK1 is a protein kinase which is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MAP2K4 / JNKK1 has been shown to activate MAPK8 / JNK1, MAPK9 / JNK2, and MAPK14 / p38, but not MAPK1 / ERK2 or MAPK3 / ERK1. MAP2K4 / JNKK1 is phosphorylated, and thus activated by MAP3K1 / MEKK. The stress-activated protein kinase (SAPK) pathways represent phosphorylation cascades that convey pro-apoptotic signals. The mitogen-activated protein kinase kinase (MAPKK) homolog MAP2K4 ( MKK4, SEK, JNKK1 ) is a centrally-placed mediator of the SAPK pathways.
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分子量
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Calculated MW: 72.0 kDa
Observed MW: 65 kDa
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UniProt
- P47809
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途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
抗原
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