IRAK4 Protein (AA 154-460)
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Quick Overview for IRAK4 Protein (AA 154-460) (ABIN7939195)
抗原
See all IRAK4 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- AA 154-460
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原理
- Recombinant IRAK4 (154-460) protein
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-460) protein (His tag)
custom-made IRAK4 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-460) protein (GST tag)IRAK4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA, KAA
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-130) protein (GST tag)IRAK4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (Active) protein (His tag)IRAK4 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by reducing SDS-PAGE. Active
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-196) protein (GST tag)IRAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant IRAK4 (154-460) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
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别名
- IRAK4
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背景
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Protein Background: IRAK4 (Interleukin-1 receptor-associated kinase 4), also known as IMD67, IPD1, IRAK-4, NY-REN-64 or REN64. is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. IRAK4 also is a Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Short Description: Recombinant IRAK4 (154-460) protein that includes amino acids 154-460 of human IRAK4 protein (accession number NP_057207.2) was expressed in a baculovirus expression system and contains an N-terminal His and FLAG tag. The molecular weight of the protein is 38.89 kDa.
Aliases: IPD1, REN64, IRAK-4, NY-REN-64
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途径
- NF-kappaB Signaling, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
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