EGF 蛋白
Quick Overview for EGF 蛋白 (ABIN7680472)
抗原
See all EGF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
质量等级
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原理
- rhEGF - Recombinant human Epidermal growth factor
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产品特性
- This product arrives as White lyophilized powder
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内毒素水平
- Less than 1 EU/mg
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Biological Activity Comment
- The ED50 is ≤1ng/ml determined by a cell proliferation assay using Balb/c 3T3 cell, corresponding to a specific activity of ≥1x10^6 Units/mg.
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Epidermal Growth Factor (EGF) (AA 974-1026) (Active) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, AcA Active
Epidermal Growth Factor (EGF) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended to reconstitute the lyophilized OsrhEGF in sterile water.
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缓冲液
- It is lyophilized with Mannitol as the stabilizer.
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注意事项
- Use as soon as possible after opening. Avoid freeze-thaw cycles
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储存条件
- -20 °C
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储存方法
- Stored at temperatures -20°C for at least 36 months. Use as soon as possible after opening. Please avoid freeze-thaw cycles.
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有效期
- 36 months
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- EGF (Epidermal Growth Factor (EGF))
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别名
- Epidermal growth factor
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背景
- Human epidermal growth factor, also known as EGF, is a 6.2kD protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface, EGF results in a variety of biochemical changes within the cell, rising intracellular calcium levels, increasing glycolysis, protein synthesis and regulating the gene expression, including the gene for EGFR that ultimately lead to DNA synthesis and cell proliferation.
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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