Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in human embryonic kidney cells (HEK293T). The ED50 for this effect is 10-40 ng/mL in the presence of 20 ng/mL of recombinant mouse Wnt-3a.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.
储存条件
4 °C,-20 °C,-80 °C
储存方法
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
有效期
12 months
抗原
SFRP2
(Secreted Frizzled-Related Protein 2 (SFRP2))