GDNF Protein (Biotin)
Quick Overview for GDNF Protein (Biotin) (ABIN7582099)
抗原
See all GDNF 蛋白蛋白类型
宿主
资源
应用范围
-
-
标记
- This GDNF protein is labelled with Biotin.
-
序列
- SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI-OH
-
特异性
- Toxin target: GFRA1 (GDNFR-Alpha-1), RET Receptor Tyrosine Kinase, LC-Biotin
-
产品特性
- human GDNF-Biotin (ABIN7582099) is a highly pure, recombinant, and biologically active conjugated protein. Benefits of human GDNF-Biotin: Localization and distribution Live cell imaging Single cell detection Indirect flow cytometry We gladly take on collaboration projects. Please Contact Us.
-
-
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein
GDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)GDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 1-211) protein (GST tag)GDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (Fc Tag)GDNF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Glial Cell Line Derived Neurotrophic Factor (GDNF) proteinGDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
-
-
应用备注
- Antigen Preadsorption Control: 1 nM - 0.1 μM
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
-
缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
-
储存条件
- -20 °C
-
储存方法
- Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
-
-
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
-
别名
- GDNF
-
背景
- Glial Cell Line-Derived Neurotrophic Factor,Glial-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET proto-oncogene and one of the four α1-α4 receptors.1 GDNF promotes survival of various neuronal cells, including motoneurons,2,3 Purkinje cells and sympathetic neurons.4 In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity DA uptake.5 Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells,6 neurons, pinealocytes, and skeletal muscle cells.7 In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from cell death.8 GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons.9,10
-
分子量
- 30.8 kDa (dimer)
-
途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
抗原
-
