SMARCA2 Protein (AA 1-1577) (His tag)
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- 抗原 See all SMARCA2 蛋白
- SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1577
- 宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This SMARCA2 protein is labelled with His tag.
- 原理
- Made-to-order recombinant Smarca2 Protein expressed in mammalian cells.
- 序列
- MSTPTDPAAM PHPGPSPGPG PSPGPILGPS PGPGPSPGSV HSMMGPSPGP PSVSHPLSTM GSADFPQEGM HQLHKPMDGI HDKGIVEDVH CGSMKGTSMR PPHPGMGPPQ SPMDQHSQGY MSPHPSPLGA PEHVSSPTPP QMPPSQPGAL IPGDPQAMNQ PNRGPSPFSP VQLHQLRAQI LAYKMLARGQ PLPETLQLAV QGKRTLPGMQ QQQQQQQQQQ QQQQQQQQQQ QQQQQPQQPQ QQAQAQPQQQ QQQQQQPALV SYNRPSGPGQ ELLLSGQSAP QKLSAPAPSG RPSPAPQAAV QPTATAVPGP SVQQPAPGQP SPVLQLQQKQ SRISPIQKPQ GLDPVEILQE REYRLQARIA HRIQELESLP GSLPPDLRTK ATVELKALRL LNFQRQLRQE VVACMRRDTT LETALNSKAY KRSKRQTLRE ARMTEKLEKQ QKIEQERKRR QKHQEYLNSI LQHAKDFKEY HRSVAGKIQK LSKAVATWHA NTEREQKKET ERIEKERMRR LMAEDEEGYR KLIDQKKDRR LAYLLQQTDE YVANLTNLVW EHKQAQAAKE KKKRRRRKKK AEENAEGGEP ALGPDGEPID ESSQMSDLPV KVTHTETGKV LFGPEAPKAS QLDAWLEMNP GYEVAPRSDS EESESDYEEE DEEEESSRQE TEEKILLDPN SEEVSEKDAK QIIETAKQDV DDEYSMQYSA RGSQSYYTVA HAISERVEKQ SALLINGTLK HYQLQGLEWM VSLYNNNLNG ILADEMGLGK TIQTIALITY LMEHKRLNGP YLIIVPLSTL SNWTYEFDKW APSVVKISYK GTPAMRRSLV PQLRSGKFNV LLTTYEYIIK DKHILAKIRW KYMIVDEGHR MKNHHCKLTQ VLNTHYVAPR RILLTGTPLQ NKLPELWALL NFLLPTIFKS CSTFEQWFNA PFAMTGERVD LNEEETILII RRLHKVLRPF LLRRLKKEVE SQLPEKVEYV IKCDMSALQK ILYRHMQAKG ILLTDGSEKD KKGKGGAKTL MNTIMQLRKI CNHPYMFQHI EESFAEHLGY SNGVINGAEL YRASGKFELL DRILPKLRAT NHRVLLFCQM TSLMTIMEDY FAFRNFLYLR LDGTTKSEDR AALLKKFNEP GSQYFIFLLS TRAGGLGLNL QAADTVVIFD SDWNPHQDLQ AQDRAHRIGQ QNEVRVLRLC TVNSVEEKIL AAAKYKLNVD QKVIQAGMFD QKSSSHERRA FLQAILEHEE ENEEEDEVPD DETLNQMIAR REEEFDLFMR MDMDRRREDA RNPKRKPRLM EEDELPSWII KDDAEVERLT CEEEEEKIFG RGSRQRRDVD YSDALTEKQW LRAIEDGNLE EMEEEVRLKK RKRRRNVDKD PVKEDVEKAK KRRGRPPAEK LSPNPPKLTK QMNAIIDTVI NYKDSSGRQL SEVFIQLPSR KDLPEYYELI RKPVDFKKIK ERIRNHKYRS LGDLEKDVML LCHNAQTFNL EGSQIYEDSI VLQSVFKSAR QKIAKEEESE EESNEEEEED DEEESESEAK SVKVKIKLNK KEEKGRDTGK GKKRPNRGKA KPVVSDFDSD EEQEENEQSE ASGTDNE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 1-1590) protein (His tag)
custom-made SMARCA2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 659-735) protein (GST tag)SMARCA2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 1367-1511) protein (GST tag)SMARCA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Bind, ScA
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 1367-1511) protein (His tag,DYKDDDDK Tag)SMARCA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Bind, ScA
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 700-1216), (partial) protein (His-SUMO Tag)SMARCA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2) (AA 700-1216), (partial) protein (His tag)SMARCA2 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))
- 别名
- Smarca2 (SMARCA2 产品)
- 背景
- Probable global transcription activator SNF2L2 (EC 3.6.4.-) (ATP-dependent helicase SMARCA2) (BRG1-associated factor 190B) (BAF190B) (Protein brahma homolog) (SNF2-alpha) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2),FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. {ECO:0000250|UniProtKB:P51531, ECO:0000269|PubMed:17640523, ECO:0000303|PubMed:22952240, ECO:0000303|PubMed:26601204}.
- 分子量
- 180.3 kDa
- UniProt
- Q6DIC0
- AlphaFold
- Q6DIC0
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