SECTM1 Protein (Fc Tag,His tag)
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- 抗原 See all SECTM1 蛋白
- SECTM1 (Secreted and Transmembrane 1 (SECTM1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This SECTM1 protein is labelled with Fc Tag,His tag.
- 原理
- Active Recombinant Human SECTM1 Protein
- 序列
- QNEGWDSPIC TEGVVSVSWG ENTVMSCNIS NAFSHVNIKL RAHGQESAIF NEVAPGYFSR DGWQLQVQGG VAQLVIKGAR DSHAGLYMWH LVGHQRNNRQ VTLEVSGAEP QSAPDTG
- 特异性
- Gln29-Gly145
- 纯度
- > 97 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human CD7 Protein at 1 μg/mL (100 μL/well) can bind SECTM1 with a linear range of 0.039-1.575 ng/mL.2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to SECTM1 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 40-50% cells will adhere specifically after 60 minutes at 37°C.
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- Discover our top product SECTM1 蛋白
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Secreted and Transmembrane 1 (SECTM1) (AA 29-145) protein (Fc Tag)
SECTM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted and Transmembrane 1 (SECTM1) (AA 69-248) protein (His-IF2DI Tag)SECTM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted and Transmembrane 1 (SECTM1) (AA 29-248) protein (GST tag)SECTM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted and Transmembrane 1 (SECTM1) (AA 29-145) protein (His tag)Crystallography grade SECTM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Secreted and Transmembrane 1 (SECTM1) (AA 29-248) protein (rho-1D4 tag)Crystallography grade SECTM1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- SECTM1 (Secreted and Transmembrane 1 (SECTM1))
- 别名
- SECTM1 (SECTM1 产品)
- 别名
- K12 Protein, secreted and transmembrane 1 Protein, SECTM1 Protein
- 背景
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Description: This protein, also known as K12, is a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein of SECTM family. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The human K12 protein has been shown to be primarily expressed in spleen, prostate, testis, small intestine, and in peripheral blood leukocytes. The K12 protein is expressed on the cell surface in such small amounts as to preclude detection. Alternatively, it may be that K12 on the cell surface is rapidly cleaved to generate a soluble K12 protein. Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. May be involved in thymocyte signaling. It had been suggested a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis. In addition, as a putative natural CD7 ligand, SECTM1/K12 may be responsible for the costimulatory role it plays in T cell activation.
Name: SECTM1,K12
- 基因ID
- 6398
- UniProt
- Q8WVN6
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