MERS-Coronavirus Spike Protein (RBD) (mFc Tag)
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- 抗原 See all MERS-Coronavirus Spike (MERS-CoV S) products
- MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- RBD
- 宿主
- Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
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资源
- HEK-293 Cells
- 标记
- This MERS-Coronavirus Spike protein is labelled with mFc Tag.
- 原理
- Active Recombinant MERS-CoV Spike RBD Protein
- 序列
- EAKPSGSVVE QAEGVECDFS PLLSGTPPQV YNFKRLVFTN CNYNLTKLLS LFSVNDFTCS QISPAAIASN CYSSLILDYF SYPLSMKSDL SVSSAGPISQ FNYKQSFSNP TCLILATVPH NLTTITKPLK YSYINKCSRL LSDDRTEVPQ LVNANQYSPC VSIVPSTVWE DGDYYRKQLS PLEGGGWLVA SGSTVAMTEQ LQMGFGITVQ YGTDTNSVCP KLEFANDTKI ASQLGNCVEY
- 特异性
- Glu367-Tyr606
- 纯度
- > 90 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human CD26 at 2μg/mL (100μL/well) can bind MERS-CoV Spike RBD with a linear range of 0.5-139.82ng/mL.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))
- 别名
- MERS-CoV Spike (MERS-CoV S 产品)
- 物质类
- Viral Protein
- 背景
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Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Name: MERS-CoV Spike RBD
- 基因ID
- 14254594
- UniProt
- K9N5Q8
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