MMP13 Protein (His tag)
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- 抗原 See all MMP13 蛋白
- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This MMP13 protein is labelled with His tag.
- 原理
- Active Recombinant Human MMP-13 Protein
- 序列
- LPLPSGGDED DLSEEDLQFA ERYLRSYYHP TNLAGILKEN AASSMTERLR EMQSFFGLEV TGKLDDNTLD VMKKPRCGVP DVGEYNVFPR TLKWSKMNLT YRIVNYTPDM THSEVEKAFK KAFKVWSDVT PLNFTRLHDG IADIMISFGI KEHGDFYPFD GPSGLLAHAF PPGPNYGGDA HFDDDETWTS SSKGYNLFLV AAHEFGHSLG LDHSKDPGAL MFPIYTYTGK SHFMLPDDDV QGIQSLYGPG DEDPNPKHPK TPDKCDPSLS LDAITSLRGE TMIFKDRFFW RLHPQQVDAE LFLTKSFWPE LPNRIDAAYE HPSHDLIFIF RGRKFWALNG YDILEGYPKK ISELGLPKEV KKISAAVHFE DTGKTLLFSG NQVWRYDDTN HIMDKDYPRL IEEDFPGIGD KVDAVYEKNG YIYFFNGPIQ FEYSIWSNRI VRVMPANSIL WC
- 特异性
- Leu20-Cys471
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured in a cell migration assay using A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >2900 pmol/min/μg.
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- Discover our top product MMP13 蛋白
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Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 104-274) protein (His tag)
MMP13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 266-471) protein (His-IF2DI Tag)MMP13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 362-471) protein (GST tag)MMP13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 104-471) protein (His tag)Crystallography grade MMP13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 105-472) protein (His tag)Crystallography grade MMP13 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
- 别名
- MMP-13 (MMP13 产品)
- 别名
- CLG3 Protein, MANDP1 Protein, Clg Protein, MMP-13 Protein, Mmp1 Protein, cb1034 Protein, mmp13 Protein, gene A Protein, xCol Protein, xcl3 Protein, MMP13 Protein, MGC108008 Protein, matrix metallopeptidase 13 Protein, matrix metallopeptidase 13a Protein, matrix metallopeptidase 13 (collagenase 3) like S homeolog Protein, matrix metallopeptidase 13 (collagenase 3) Protein, matrix metalloproteinase 13 Protein, matrix metallopeptidase 13 (collagenase 3) like L homeolog Protein, MMP13 Protein, Mmp13 Protein, mmp13a Protein, mmp13l.S Protein, mmp13 Protein, LOC100136348 Protein, mmp13l.L Protein
- 背景
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Description: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases ,it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.
Name: CLG3, MANDP1, MDST, MMP-13,MMP13,MANDP1,MDST,MMP-13
- 基因ID
- 4322
- UniProt
- P45452
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