Glycerol Kinase Protein (GK) (AA 2-502) (His tag)
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- 抗原 See all Glycerol Kinase (GK) 蛋白
- Glycerol Kinase (GK)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 2-502
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宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Glycerol Kinase protein is labelled with His tag.
- 序列
- Thr2-Glu502
- 产品特性
- A DNA sequence encoding the Escherichia coli Glycerol kinase (2T-502E) was expressed with a polyhistidine tag at the N-terminus and C-terminus.
- 纯度
- >90 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to transfer phosphate from ATP to glycerol. The specific activity is ≥ 200U/mg protein, as measured under the described conditions.
- Top Product
- Discover our top product GK 蛋白
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Glycerol Kinase (GK) (AA 2-94) protein (GST tag)
GK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glycerol Kinase (GK) (AA 1-524) protein (GST tag)GK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glycerol Kinase (GK) (AA 1-559) protein (Strep Tag)Crystallography grade GK 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Glycerol Kinase (GK) (AA 1-559) protein (His tag)Crystallography grade GK 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycerol Kinase (GK) (AA 2-502) protein (His tag)GK 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Glycerol Kinase (GK) (AA 1-496), (full length) protein (His tag)GK 宿主: Thermus thermophilus 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Glycerol Kinase (GK) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Glycerol Kinase (GK) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)GK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
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- 抗原
- Glycerol Kinase (GK)
- 别名
- Glycerol kinase (GK 产品)
- 别名
- GK1 Protein, GKD Protein, D930012N15Rik Protein, GK Protein, CG18374 Protein, Dmel\\CG18374 Protein, dGyk Protein, ASTP Protein, Gyk Protein, gk1 Protein, gk2 Protein, gkd Protein, gyk Protein, Gk Protein, BmGK Protein, DKFZp469P1225 Protein, glycerol kinase Protein, Glycerol kinase 1 Protein, glycerol kinase S homeolog Protein, putative glycerol kinase 3 Protein, ATP:glycerol 3-phosphotransferase; glycerokinase; GK Protein, Glycerol kinase Protein, Fanconi anemia core complex associated protein 100 Protein, GK Protein, Gk Protein, Gk1 Protein, gk.S Protein, LOC705001 Protein, glpK Protein, Spico_1557 Protein, Trebr_0397 Protein, Halhy_5315 Protein, gk Protein, FAAP100 Protein
- 背景
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Background: Glycerol kinase from E. coli (glpK) catalyzes the ATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate (G3P), the first and rate-limiting step in the utilization of glycerol. In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator. In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6-bisphosphate (FBP) of the glycolytic pathway. Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer. FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway. GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70. Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism. The enzyme activity was measured using a phosphatase-coupled kinase assay.
Synonym: GK, glpK
- 分子量
- 56.1 kDa
- UniProt
- P0A6F3
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