PBK Protein (AA 1-322) (His tag)
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- 抗原 See all PBK 蛋白
- PBK (PDZ Binding Kinase (PBK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-322
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This PBK protein is labelled with His tag.
- 原理
- Human PBK/TOPK Protein
- 序列
- Met1-Val322
- 产品特性
- Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Val322.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product PBK 蛋白
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PDZ Binding Kinase (PBK) (AA 1-322) (Active) protein (GST tag)
PBK 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
PDZ Binding Kinase (PBK) (AA 1-322) (Active) protein (His-GST)PBK 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
PDZ Binding Kinase (PBK) (AA 1-322) protein (His tag)PBK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
PDZ Binding Kinase (PBK) (AA 1-322) protein (GST tag)PBK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
PDZ Binding Kinase (PBK) (AA 1-110) protein (GST tag)PBK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
PDZ Binding Kinase (PBK) (AA 122-229) protein (GST tag)PBK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
PDZ Binding Kinase (PBK) protein (Myc-DYKDDDDK Tag)PBK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PBK (PDZ Binding Kinase (PBK))
- 别名
- PBK (PBK 产品)
- 别名
- PBK Protein, topk Protein, CT84 Protein, Nori-3 Protein, SPK Protein, TOPK Protein, 2810434B10Rik Protein, AW538537 Protein, D14Ertd732e Protein, zgc:92050 Protein, PDZ binding kinase Protein, PDZ binding kinase L homeolog Protein, PBK Protein, pbk Protein, Pbk Protein, pbk.L Protein
- 背景
- T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1.
- 分子量
- 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
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