IGFBP7
(Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This IGFBP7 protein is labelled with His tag.
序列
Ser27-Leu282
产品特性
Recombinant Human IGFBP-7 Protein Protein is expressed from Expi293 with His tag at the N-terminal.It contains Ser27-Leu282.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IGFBP-7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD93, hFc Tag with the EC50 of 0.74μg/ml determined by ELISA. See testing image for detail.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
抗原
IGFBP7
(Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
IGFBP-7, IBP7, AGM, FSTL2, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF. IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
分子量
27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.