HCV Core/NS3/NS4 Recombinant,HCV Core/NS3/NS4 Recombinant Hepatitis C Virus (HCV) Antigen, Core/NS3/NS4, Recombinant Contains three antigens expressed in E. coli in a chimera: -HCV Core Fragment (a.a. 8 to 56) -HCV NS4 Fragment (a.a. 1916~1947) Immune-Dominant Region -HCV NS3 Fragment (a.a. 1192~1457)
纯化方法
> 95% pure (SDS-PAGE). Q-Sepharose FF, S-Sepharose FF, and Sephadex G-50.
Prior to Lyophilization: 25 mM Tris-HCl, pH 8.5 containing 0.02 % SDS. Reconstitute in distilled water. Avoid solvents containing potassium ions. NOTE: The buffer prior to lyophilization contained Tris and SDS. It is recommended to reconstitute the lyophilized antigen in distilled water. The distilled water must not have potassium ions because SDS and potassium ions generate flocculent precipitate. Tris can be removed by dialysis. We recommend adding 0.02 % SDS to the buffer used for dialysis. SDS is mandatory for recombinant antigens to dissolve, but the content is very low and will not affect avidin connection., No Preservative
储存液
Without preservative
储存条件
4 °C,-20 °C
储存方法
Store desiccated at -20°C. After reconstitution, store at 2-8°C for 3 days. Long term stability can only be achieved with lyophilized form. Inactivation: Not Applicable