SARS-CoV Spike Protein (RBD) (His-Avi Tag)
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- 抗原 See all SARS-CoV Spike (SARS-CoV S) 蛋白
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- 蛋白类型
- Recombinant
- 产品特性
- RBD
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宿主
- SARS Coronavirus (SARS-CoV)
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV Spike protein is labelled with His-Avi Tag.
- 原理
- SARS Spike RBD Protein
- 序列
- Arg306-Phe527
- 产品特性
- Recombinant SARS Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. See testing image for detail.
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SARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (His tag)
SARS-CoV S 宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS. ELISA, SDSSARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (His-Avi Tag,Biotin)SARS-CoV S 宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-Coronavirus Spike Protein (SARS-CoV S) (RBD) protein (mFc Tag)SARS-CoV S 宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE.
SARS-Coronavirus Spike Protein (SARS-CoV S) (AA 14-1195) (Active) protein (His tag)SARS-CoV S 宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- 别名
- SARS-CoV Spike (SARS-CoV S 产品)
- 背景
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.
- UniProt
- P59594
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