ROR2 Protein (AA 34-403) (hFc Tag)
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Quick Overview for ROR2 Protein (AA 34-403) (hFc Tag) (ABIN7275531)
抗原
See all ROR2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 34-403
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标记
- This ROR2 protein is labelled with hFc Tag.
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原理
- Human ROR2/NTRKR2 Protein
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序列
- Val34-Gly403
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产品特性
- Recombinant Human ROR2/NTRKR2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val34-Gly403.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human ROR2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ROR2 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.,Human ROR2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of- Human ROR2 is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 24 months
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- ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))
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别名
- ROR2
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背景
- ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system. Mature human ROR2 contains a 369 amino acid (aa) extracellular domain (ECD) and a 518 aa cytoplasmic tail containing an tyrosine kinase domain.
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分子量
- 68 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
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UniProt
- A1L4F5
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途径
- RTK signaling, WNT signaling
抗原
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