RNF34 Protein (AA 24-197) (Fc Tag)
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- 抗原 See all RNF34 蛋白
- RNF34 (Ring Finger Protein 34 (RNF34))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-197
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This RNF34 protein is labelled with Fc Tag.
- 原理
- Mouse RNF43 Protein
- 序列
- Gly24-Tyr197
- 产品特性
- Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
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Ring Finger Protein 34 (RNF34) (AA 24-197) protein (Fc Tag)
RNF34 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Ring Finger Protein 34 (RNF34) (AA 1-373) protein (His tag)RNF34 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (GST tag)RNF34 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Ring Finger Protein 34 (RNF34) (AA 1-372) protein (Strep Tag)Crystallography grade RNF34 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ring Finger Protein 34 (RNF34) (AA 1-376) protein (Strep Tag)Crystallography grade RNF34 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ring Finger Protein 34 (RNF34) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)RNF34 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- RNF34 (Ring Finger Protein 34 (RNF34))
- 别名
- RNF34 (RNF34 产品)
- 别名
- CARP-1 Protein, CARP1 Protein, RFI Protein, RIF Protein, RIFF Protein, hRFI Protein, rnf34 Protein, AW061037 Protein, AW536122 Protein, BC004042 Protein, C88279 Protein, Momo Protein, fi35g06 Protein, wu:fi35g06 Protein, zgc:114043 Protein, zgc:171755 Protein, ring finger protein 34 Protein, E3 ubiquitin-protein ligase RNF34 Protein, ring finger protein 34a Protein, ring finger protein 34b Protein, Residual feed intake Protein, RNF34 Protein, rnf34 Protein, Rnf34 Protein, rnf34a Protein, rnf34b Protein, RFI Protein
- 背景
- RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
- 分子量
- 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q5NCP0
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