Recombinant Cynomolgus PSMA/FOLH1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys44-Ala750.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus PSMA, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA. See testing image for detail.
FOLH1
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
分子量
80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.