Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 100 mM NaCl ( pH 6.0).
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM MES, 100 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
C14orf175 Protein, AI132321 Protein, thss Protein, phospholipase D family member 4 Protein, phospholipase D family, member 4 Protein, PLD4 Protein, Pld4 Protein
背景
Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages.
分子量
51 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.