PD-L1 Protein (AA 19-238) (mFc Tag)
Quick Overview for PD-L1 Protein (AA 19-238) (mFc Tag) (ABIN7275431)
抗原
See all PD-L1 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 19-238
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标记
- This PD-L1 protein is labelled with mFc Tag.
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原理
- Human PD-L1/B7-H1 Protein
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序列
- Phe19-Arg238
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产品特性
- Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Phe19-Arg238.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human PD-L1, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 0.9μg/ml determined by ELISA. See testing image for detail.
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CD274 (PD-L1) (AA 19-238) protein (Fc Tag)
PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His-Avi Tag,Biotin)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag)PD-L1 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (mFc-His Tag)PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag)HPLC verified PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
CD274 (PD-L1) (AA 19-238) protein (Fc Tag)PD-L1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-237) protein (Fc Tag)PD-L1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag)PD-L1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag,Biotin)PD-L1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- PD-L1 (CD274 (PD-L1))
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别名
- PD-L1
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背景
- B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
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分子量
- 50.9 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9NZQ7-1
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途径
- Cancer Immune Checkpoints
抗原
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