Recombinant Human OSCAR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp19-Ser229.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human OSCAR, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-OSCAR Antibody, hFc Tag with the EC50 19.2ng/ml determined by ELISA. See testing image for detail.
OSCAR
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
OSCAR Protein, mOSCAR-M3 Protein, mOSCAR Protein, mOSCAR-M1 Protein, mOSCAR-M2 Protein, PIGR3 Protein, PIgR-3 Protein, RGD1559897 Protein, osteoclast associated, immunoglobulin-like receptor Protein, osteoclast-associated immunoglobulin-like receptor Protein, osteoclast associated receptor Protein, OSCAR Protein, LOC748876 Protein, Oscar Protein
背景
Osteoclast-associated receptor (OSCAR) is a co-stimulatory receptor in osteoclastogenesis. Synovial tissues from active rheumatoid arthritis (RA) patients express higher levels of OSCAR compared with osteoarthritic and normal patients. OSCAR and tartrate-resistant acid phosphatase (TRAP) expression levels did not differ between the cartilage pannus junction (CPJ) and non-CPJ regions in active RA.
分子量
49.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.