Recombinant Cynomolgus NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro22-Asn254.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus NKp46, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
NCR1
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl, 100 mM L-arginine ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
分子量
27.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.