MMP8 Protein (AA 21-467) (His tag)
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- 抗原 See all MMP8 蛋白
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 21-467
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This MMP8 protein is labelled with His tag.
- 原理
- Human MMP-8 Protein
- 序列
- Phe21-Gly467
- 产品特性
- Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Gly467.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5μg/ml determined by ELISA. The affinity constant of 1.14 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)
MMP8 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-465) protein (His tag)MMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 1-467) protein (GST tag)MMP8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) proteinMMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
- 别名
- MMP-8 (MMP8 产品)
- 别名
- MMP8 Protein, BB138268 Protein, CLG1 Protein, HNC Protein, MMP-8 Protein, PMNL-CL Protein, matrix metallopeptidase 8 Protein, neutrophil collagenase Protein, MMP8 Protein, Mmp8 Protein, LOC100339790 Protein
- 背景
- Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.
- 分子量
- 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P22894
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