MFAP4
(Microfibrillar-Associated Protein 4 (MFAP4))
蛋白类型
Recombinant
产品特性
AA 22-255
宿主
人
资源
HEK-293 Cells
标记
This MFAP4 protein is labelled with His-DYKDDDDK Tag.
原理
Human MFAP4 Protein
序列
Val22-Ala255
产品特性
Recombinant Human MFAP4 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val22-Ala255.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human MFAP4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
MFAP4
(Microfibrillar-Associated Protein 4 (MFAP4))
1110007F23Rik Protein, Magp-36 Protein, zgc:77076 Protein, microfibril-associated glycoprotein 4 Protein, microfibrillar-associated protein 4 Protein, microfibril associated protein 4 S homeolog Protein, microfibril associated protein 4 Protein, CpipJ_CPIJ000434 Protein, CpipJ_CPIJ006120 Protein, CpipJ_CPIJ010089 Protein, CpipJ_CPIJ018551 Protein, CpipJ_CPIJ020296 Protein, mfap4 Protein, mfap4.S Protein, MFAP4 Protein, Mfap4 Protein
背景
Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease.
分子量
28.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.