LRRC15 Protein (AA 22-538) (His-Avi Tag,Biotin)
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- 抗原 See all LRRC15 products
- LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-538
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宿主
- 人
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资源
- CHO Cells
- 标记
- This LRRC15 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human LRRC15/LIB Protein
- 序列
- Tyr22-Gly538
- 产品特性
- Recombinant Biotinylated Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-LRRC15 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 38.3ng/ml determined by ELISA. See testing image for detail.
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Leucine Rich Repeat Containing 15 (LRRC15) protein (His-Avi Tag)
LRRC15 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Leucine Rich Repeat Containing 15 (LRRC15) (AA 22-536) protein (His tag)LRRC15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leucine Rich Repeat Containing 15 (LRRC15) (AA 22-538) protein (His tag)LRRC15 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Leucine Rich Repeat Containing 15 (LRRC15) (AA 47-565) protein (His tag)LRRC15 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))
- 别名
- LRRC15 (LRRC15 产品)
- 别名
- cb894 Protein, zgc:158286 Protein, LIB Protein, 5430427N11Rik Protein, Lib Protein, leucine rich repeat containing 15 Protein, leucine-rich repeat-containing protein 15 Protein, carboxypeptidase N subunit 2 Protein, LRRC15 Protein, lrrc15 Protein, CpipJ_CPIJ004946 Protein, CpipJ_CPIJ005191 Protein, CpipJ_CPIJ007745 Protein, CPN2 Protein, Lrrc15 Protein
- 背景
- The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
- 分子量
- 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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