LRP6 Protein (AA 20-630) (mFc Tag)
This Recombinant LRP6 protein is expressed in HEK-293 Cells.
Quick Overview for LRP6 Protein (AA 20-630) (mFc Tag) (ABIN7275210)
抗原
See all LRP6 蛋白
LRP6
(Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 90 % as determined by Tris-Bis PAGE
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产品特性
- AA 20-630
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标记
- This LRP6 protein is labelled with mFc Tag.
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原理
- Human LRP-6 Protein
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序列
- Ala20-Pro630
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产品特性
- Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human DKK1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.1μg/ml determined by ELISA. See testing image for detail.
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Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (AA 20-1370) protein (His tag)
LRP6 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (AA 20-630) (Active) protein (Fc Tag)LRP6 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
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别名
- LRP-6
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背景
- Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.
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分子量
- 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
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UniProt
- O75581-1
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途径
- WNT signaling, Tube Formation
抗原
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