Recombinant Human IL-22R alpha 1 & IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-22, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-22R alpha 1&IL-10R beta, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL-22R alpha 1 & IL-10R beta
背景
IL-22Rα1 & IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL--22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 Rβ or IL-20 Rβ and the respective ligands is required for signal transduction.
分子量
52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.