IL18RAP
(Interleukin 18 Receptor Accessory Protein (IL18RAP))
蛋白类型
Recombinant
产品特性
AA 20-356
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This IL18RAP protein is labelled with His tag.
原理
Cynomolgus IL-18RAP Protein
序列
Phe20-Arg356
产品特性
Recombinant Cynomolgus IL-18RAP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Arg356.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus IL-18RAP, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL18RAP
(Interleukin 18 Receptor Accessory Protein (IL18RAP))
IL18RAP Protein, ACPL Protein, CD218b Protein, CDw218b Protein, IL18RB Protein, interleukin 18 receptor accessory protein Protein, IL18RAP Protein, Il18rap Protein
背景
IL-18RAP, also known as IL-18Rβ or IL-1R7, is component of IL-18 receptor complex and its interactions with IL-18R1 mediate signal transduction initiated by IL-18 although it does not directly bind IL-18 in vitro.
分子量
39.5 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.