HGF Protein (AA 32-728) (His tag)
Quick Overview for HGF Protein (AA 32-728) (His tag) (ABIN7274808)
抗原
See all HGF 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 32-728
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标记
- This HGF protein is labelled with His tag.
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原理
- Human HGF Protein
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序列
- Gln32-Ser728
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产品特性
- Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Ser728.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human HGF, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 46.7ng/ml determined by ELISA. See testing image for detail.
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Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (Fc Tag)
HGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-494), (AA 495-728) (Active) proteinHGF 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-285) proteinHGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 496-728) protein (His-IF2DI Tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 493-721) protein (His-IF2DI Tag)HGF 宿主: 小鸡 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 619-728) protein (GST tag)HGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (His tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (Fc Tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 477-728) protein (GST tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
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别名
- HGF
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背景
- Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
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分子量
- the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
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UniProt
- P14210-1
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途径
- RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
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