GPA33 Protein (His-Avi Tag,Biotin)
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- 抗原 See all GPA33 蛋白
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This GPA33 protein is labelled with His-Avi Tag,Biotin.
- 序列
- Ile22-Val235
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail.
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Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His-Avi Tag)
GPA33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag)GPA33 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein A33 (Transmembrane) (GPA33) protein (Fc Tag)GPA33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glycoprotein A33 (Transmembrane) (GPA33) (AA 1-319) protein (GST tag)GPA33 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glycoprotein A33 (Transmembrane) (GPA33) (AA 26-125) protein (GST tag)GPA33 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag)Crystallography grade GPA33 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag)Crystallography grade GPA33 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-319) protein (rho-1D4 tag)Crystallography grade GPA33 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-319) protein (rho-1D4 tag)Crystallography grade GPA33 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glycoprotein A33 (Transmembrane) (GPA33) (AA 22-235) protein (His tag,Biotin)GPA33 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))
- 别名
- GPA33 (GPA33 产品)
- 别名
- A33 Protein, 2010310L10Rik Protein, 2210401D16Rik Protein, BB116197 Protein, glycoprotein A33 Protein, glycoprotein A33 (transmembrane) Protein, GPA33 Protein, Gpa33 Protein
- 背景
- Glycoprotein A33, GPA33, A33, MGC129986, MGC129987,Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
- 分子量
- 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
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