GFRA1 Protein (AA 25-424) (His tag)
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- 抗原 See all GFRA1 蛋白
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-424
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This GFRA1 protein is labelled with His tag.
- 原理
- Human GFR alpha 1 Protein
- 序列
- Asp25-Ser424
- 产品特性
- Recombinant Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Ser424.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
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GDNF Family Receptor alpha 1 (GFRA1) (AA 267-360) protein (GST tag)
GFRA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (AA 1-460) protein (GST tag)GFRA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (Fc Tag)GFRA1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (AA 25-429) protein (His tag)Crystallography grade GFRA1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
GDNF Family Receptor alpha 1 (GFRA1) (AA 25-430) protein (His tag)Crystallography grade GFRA1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- 别名
- GFR alpha 1 (GFRA1 产品)
- 别名
- gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein
- 背景
- Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
- 分子量
- 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- NCBI登录号
- NP_665736
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