This GDF15 protein is labelled with Fc Tag,Biotin.
原理
Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling)
序列
Ala197-Val308
产品特性
Recombinant Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala197-Val308.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus GDF15, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
GDF15
宿主: 大鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by Tris-Bis PAGE
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
分子量
37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.