FLT3 Protein (AA 28-541) (Fc Tag)
Quick Overview for FLT3 Protein (AA 28-541) (Fc Tag) (ABIN7274655)
抗原
See all FLT3 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 28-541
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标记
- This FLT3 protein is labelled with Fc Tag.
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原理
- Cynomolgus FLT3/Flk-2 Protein
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序列
- Gln28-Ser541
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产品特性
- Recombinant Cynomolgus FLT3/Flk-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln28-Ser541.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human FLT3 Ligand, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus FLT3, hFc Tag with the EC50 of 18ng/ml determined by ELISA. See testing image for detail.
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Fms-Related tyrosine Kinase 3 (FLT3) (AA 27-541) protein (Fc Tag)
FLT3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fms-Related tyrosine Kinase 3 (FLT3) (AA 28-544) protein (Fc Tag)FLT3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fms-Related tyrosine Kinase 3 (FLT3) (AA 27-543) protein (Fc-His Tag)FLT3 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Fms-Related tyrosine Kinase 3 (FLT3) protein (His tag)FLT3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 75 % as determined by SDS-PAGE
Fms-Related tyrosine Kinase 3 (FLT3) (AA 27-543) (Active) protein (Fc Tag)FLT3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
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别名
- FLT3
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背景
- The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
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分子量
- 84.58 kDa. Due to glycosylation, the protein migrates to 160-180 kDa based on Tris-Bis PAGE result.
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UniProt
- A0A2K5VQ48
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途径
- RTK signaling
抗原
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