Fc gamma RII (CD32) (AA 32-215) protein (His tag)
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- 抗原 See all Fc gamma RII (CD32) 蛋白
- Fc gamma RII (CD32)
- 蛋白类型
- Recombinant
- 产品特性
- AA 32-215
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- His tag
- 原理
- Mouse Fc gamma RIII/CD16 Protein
- 序列
- Leu32-Thr215
- 产品特性
- Recombinant Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu32-Thr215.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.194 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fc gamma RII (CD32) (AA 17-208) protein (His tag)
CD32 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc gamma RII (CD32) (AA 34-216) protein (His tag)CD32 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Fc gamma RII (CD32) (AA 30-210) protein (His tag)Crystallography grade CD32 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fc gamma RII (CD32) (AA 30-330) protein (rho-1D4 tag)Crystallography grade CD32 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fc gamma RII (CD32) (AA 47-285) protein (His tag,T7 tag)CD32 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
Fc gamma RII (CD32) (AA 69-309) protein (His tag,T7 tag)CD32 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
Fc gamma RII (CD32) proteinCD32 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
Fc gamma RII (CD32) proteinCD32 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % ELISA, WB, SDS, IP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Fc gamma RII (CD32)
- 别名
- Fc gamma RIII (CD32 产品)
- 别名
- CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc receptor, IgG, low affinity IIb Protein, FCGR2B Protein, Fcgr2b Protein
- 背景
- Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R, Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
- 分子量
- 22.2 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q5D5I8
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