Recombinant Human FCRL2/IRTA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Val401.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human FCRL2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FCRL2 Antibody, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA. See testing image for detail.
FCRL2
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD307b Protein, FCRH2 Protein, IFGP4 Protein, IRTA4 Protein, SPAP1 Protein, SPAP1A Protein, SPAP1B Protein, SPAP1C Protein, fcrh2 Protein, ifgp4 Protein, irta4 Protein, spap1 Protein, spap1a Protein, spap1b Protein, spap1c Protein, Fc receptor like 2 Protein, Fc receptor-like 2 Protein, Fc receptor-like protein 2 Protein, Fc receptor-like 2 L homeolog Protein, FCRL2 Protein, LOC612038 Protein, fcrl2.L Protein
背景
Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation, P < 0.0001) and cells of other hematological diseases.
分子量
42.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.