EGFRviii Protein (AA 25-378) (His-Avi Tag)
Quick Overview for EGFRviii Protein (AA 25-378) (His-Avi Tag) (ABIN7274517)
抗原
蛋白类型
宿主
资源
纯度
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产品特性
- AA 25-378
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标记
- This EGFRviii protein is labelled with His-Avi Tag.
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原理
- Human EGFRVIII Protein
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序列
- Leu25-Ser378
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产品特性
- Recombinant Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu25-Ser378.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.
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EGFR vIII (EGFRv3) (AA 25-378) protein (His-Avi Tag,Biotin)
EGFRv3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EGFR vIII (EGFRv3) (AA 25-378) protein (FITC,His-Avi Tag)EGFRv3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
EGFR vIII (EGFRv3) (AA 25-645) protein (His tag)EGFRv3 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
EGFR vIII (EGFRv3) (AA 25-378) protein (PE,His-Avi Tag)EGFRv3 宿主: 人 宿主: HEK-293 Cells Recombinant
EGFR vIII (EGFRv3) (AA 25-378) (Active) protein (His tag,FITC)EGFRv3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
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别名
- EGFRVIII
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背景
- The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
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分子量
- 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
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NCBI登录号
- NP_001333870
抗原
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