CD40 Ligand Protein (CD40LG) (AA 113-261, Trimer) (Fc Tag)
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- 抗原 See all CD40 Ligand (CD40LG) 蛋白
- CD40 Ligand (CD40LG)
- 蛋白类型
- Recombinant
- 产品特性
- AA 113-261, Trimer
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD40 Ligand protein is labelled with Fc Tag.
- 原理
- Human CD40 Ligand/TNFSF5 Trimer Protein
- 序列
- Met113-Leu261
- 产品特性
- Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13μg/ml determined by ELISA. See testing image for detail.
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag,Biotin)
CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 1-260) protein (His tag)custom-made CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 1-261) protein (His tag)custom-made CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) protein (Fc Tag)CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG 宿主: 人, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE.
CD40 Ligand (CD40LG) (Active) protein (His tag)CD40LG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD40 Ligand (CD40LG)
- 别名
- CD40 Ligand (CD40LG 产品)
- 背景
- CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. .
- 分子量
- 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
- UniProt
- P29965
- 途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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