CD40 Ligand Protein (CD40LG) (His tag)
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Quick Overview for CD40 Ligand Protein (CD40LG) (His tag) (ABIN7320848)
抗原
See all CD40 Ligand (CD40LG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD40 Ligand protein is labelled with His tag.
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原理
- Recombinant Mouse CD40LG/TNFSF5 Protein (His Tag)(Active)
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序列
- Met112-Leu260
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产品特性
- Recombinant Mouse CD40L is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human CD40-Fc(Cat: PKSM041225) at 2μg/ml(100 μl/well) can bind Human CD40LG-His. The ED50 of Human CD40LG-His(Cat: PKSH033600)) is 13.84ng/mL.
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (Fc Tag)
CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (Biotin,His-DYKDDDDK Tag)CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) (Active) protein (His-DYKDDDDK Tag)CD40LG 宿主: 人, 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) (Active) protein (Fc Tag)CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG 宿主: 人, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) protein (Biotin,His-Avi Tag)CD40LG 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
CD40 Ligand (CD40LG) proteinCD40LG 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD40 Ligand (CD40LG)
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别名
- CD40LG/TNFSF5
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背景
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Background: CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Synonym: CD40 Ligand, CD40LG,HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
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分子量
- 18.8 kDa
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UniProt
- P27548
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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