CHRDL1 Protein (AA 28-456) (His tag)
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- 抗原 See all CHRDL1 蛋白
- CHRDL1 (Chordin-Like 1 (CHRDL1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 28-456
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CHRDL1 protein is labelled with His tag.
- 原理
- Human CHRDL1 Protein
- 序列
- Glu28-Cys456
- 产品特性
- Recombinant Human CHRDL1 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Glu28-Cys456.
- 纯度
- > 90 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.
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Chordin-Like 1 (CHRDL1) (AA 1-450) protein (GST tag)
CHRDL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chordin-Like 1 (CHRDL1) (AA 347-456) protein (GST tag)CHRDL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chordin-Like 1 (CHRDL1) (AA 23-447) protein (His tag)Crystallography grade CHRDL1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Chordin-Like 1 (CHRDL1) (AA 22-450) protein (His tag)Crystallography grade CHRDL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CHRDL1 (Chordin-Like 1 (CHRDL1))
- 别名
- CHRDL1 (CHRDL1 产品)
- 别名
- CHL Protein, NRLN1 Protein, VOPT Protein, dA141H5.1 Protein, CHL1 Protein, Nrln1 Protein, chl Protein, vopt Protein, nrln1 Protein, da141h5.1 Protein, Kjr Protein, Neurogenesin-1 Protein, Ng1 Protein, chordin like 1 Protein, chordin-like 1 Protein, CHRDL1 Protein, Chrdl1 Protein, chrdl1 Protein
- 背景
- CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis.The hypermethylation of the CHRDL1 promoter in gastric cancer, which induced low expression of CHRDL1 and decreased its secretion to the supernatant.
- 分子量
- 50 kDa same as Tris-Bis PAGE result.
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