This CDCP1 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Cynomolgus CDCP1 Protein
序列
Ala29-Thr667
产品特性
Recombinant Biotinylated Cynomolgus CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Thr667.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CDCP1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CDCP1, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
CDCP1
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 79 % as determined by SDS-PAGE
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
分子量
75 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.