CD3 epsilon Protein (CD3E) (AA 23-126) (Fc Tag)
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- 抗原 See all CD3 epsilon (CD3E) 蛋白
- CD3 epsilon (CD3E)
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-126
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD3 epsilon protein is labelled with Fc Tag.
- 原理
- Human CD3E/CD3 epsilon Protein
- 序列
- Asp23-Asp126(C119S, C122S)
- 产品特性
- Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S).
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product CD3E 蛋白
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CD3 epsilon (CD3E) (AA 22-117) protein (Fc Tag)
CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-117) protein (His tag)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-126) protein (His tag)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag,Biotin)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag,Biotin)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (Active) protein (mFc Tag)CD3E 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD3 epsilon (CD3E) (AA 23-126) protein (His tag,AVI tag,Biotin)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
CD3 epsilon (CD3E) (AA 23-207) protein (GST tag)CD3E 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 背景
- T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- 分子量
- 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.
- UniProt
- P07766
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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