VTCN1
(V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This VTCN1 protein is labelled with His-Avi Tag,Biotin.
序列
Phe29-Ala258
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-B7-H4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H4, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.
VTCN1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 90 % by SDS-PAGE gel analyses
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
VTCN1
(V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein
背景
B7H4, B7-H4, B7S1, B7h.5, B7x, FLJ22418, VTCN1, PRO1291,B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
分子量
28.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.