ARTN Protein (AA 108-220) (Fc Tag)
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- 抗原 See all ARTN 蛋白
- ARTN (Artemin (ARTN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 108-220
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ARTN protein is labelled with Fc Tag.
- 原理
- Human ARTN Protein
- 序列
- Ala108-Gly220
- 产品特性
- Recombinant Human ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala108-Gly220.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GFRA3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA. See testing image for detail.
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Artemin (ARTN) (AA 112-224) protein (Fc Tag)
ARTN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Artemin (ARTN) (AA 108-220) protein (His tag)ARTN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Artemin (ARTN) (AA 1-228) protein (GST tag)ARTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl ( pH 2.8).
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 4 mM HCl ( pH 2.8). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ARTN (Artemin (ARTN))
- 别名
- ARTN (ARTN 产品)
- 别名
- ARTN Protein, ENOVIN Protein, EVN Protein, NBN Protein, neublastin Protein, artemin Protein, ARTN Protein, Artn Protein
- 背景
- Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
- 分子量
- 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
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