This Alkaline Phosphatase protein is labelled with His tag.
原理
Mouse Alkaline phosphatase (Intestinal type) /ALPI Protein
序列
Ile21-Gly501
产品特性
Recombinant Mouse Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Gly501.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
分子量
53.10 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.