ADAM8 Protein (AA 17-655) (His tag)
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- 抗原 See all ADAM8 蛋白
- ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-655
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ADAM8 protein is labelled with His tag.
- 原理
- Human ADAM8 Protein
- 序列
- Ile17-Pro655
- 产品特性
- Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Pro655.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ADAM8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
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ADAM Metallopeptidase Domain 8 (ADAM8) (AA 17-658) protein (His tag)
ADAM8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
ADAM Metallopeptidase Domain 8 (ADAM8) (AA 188-373) protein (His-IF2DI Tag)ADAM8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
ADAM Metallopeptidase Domain 8 (ADAM8) (AA 17-497) protein (His tag)ADAM8 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))
- 别名
- ADAM8 (ADAM8 产品)
- 背景
- A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and 'dispensable' ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer.
- 分子量
- 70.9 kDa. The protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P78325
- 途径
- Activation of Innate immune Response, M Phase
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