HLA DQA1*01:01-DQB1*06:02 protein (V5 tag,SBP Tag,His tag)
宿主: 人
宿主: Mammalian Cells
Recombinant
> 80 %
BP, CP, ELISA, Neut
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- 抗原
- HLA DQA1*01:01-DQB1*06:02
- 蛋白类型
- Recombinant
- 宿主
- 人
- 资源
- Mammalian Cells
- 标记
- V5 tag,SBP Tag,His tag
- 应用范围
- Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)
- 原理
- The product is HLA DQA1*01:01-DQB1*06:02 as zipper-stabilized heterodimer
- 序列
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alpha chain: EDIVADHVAS YGVNLYQSYG PSGQFTHEFD GDEEFYVDLE RKETVWKLPL FHRLRFDPQF ALTNIAVLKH NLNILIKRSN STAATNEVPE VTVFSKSPVT LGQPNTLICL VDNIFPPVVN ITWLSNGHSV TEGVSETSFL SKSDHSFFKI SYLTFLPSAD EIYDCKVEHW GLDEPLLKHW EPEIPAPMSE LTET
beta chain: RDSPEDFVFQ FKGMCYFTNG TERVRLVTRY IYNREEYARF DSDVGVYRAV TPQGRPDAEY WNSQKEVLEG TRAELDTVCR HNYEVAFRGI LQRRVEPTVT ISPSRTEALN HHNLLVCSVT DFYPGQIKVR WFRNDQEETA GVVSTPLIRN GDWTFQILVM LEMTPQRGDV YTCHVEHPSL QSPITVEWRA QSESAQSK
Sequences without tags. Tag location is at the discretion of the manufacturer. - 特异性
- HLA DQA1*01:01-DQB1*06:02, P01909 / P01920
- 产品特性
- HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.
- 纯化方法
- Chromatography
- 纯度
- > 80 %
- 内毒素水平
- Endotoxins have not been removed
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- 应用备注
- ELISA: 10 - 1000 ng/mL
- 说明
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sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Phosphate based buffer, pH 7.8, Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原
- HLA DQA1*01:01-DQB1*06:02
- 背景
- Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.
- 分子量
- approx. 60 - 65 kDa (approx. 29 kDa alpha chain and 34 - 38 kDa beta chain)
- UniProt
- P01909
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