SEBOV GP Protein (His tag,Mucin Tag)
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- 抗原 See all SEBOV GP products
- SEBOV GP (Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP))
- 蛋白类型
- Recombinant
- 宿主
- Ebola Virus
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资源
- HEK-293 Cells
- 标记
- This SEBOV GP protein is labelled with His tag,Mucin Tag.
- 原理
- Recombinant EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP1 (mucin domain deleted) Protein (His Tag)
- 序列
- Met1-Asp320
- 产品特性
- A DNA sequence encoding Sudan ebola virus (strain Uganda-00) GP (Q7T9D9) (Met1-Asp320) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
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Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP) protein (His tag)
SEBOV GP 宿主: Ebola Virus 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP) protein (Fc Tag)SEBOV GP 宿主: Ebola Virus 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE.
Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP) protein (His tag,Mucin Tag)SEBOV GP 宿主: Ebola Virus 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE.
Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP) protein (His tag)SEBOV GP 宿主: Ebola Virus 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
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- 说明
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46-64 kDa
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- SEBOV GP (Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP))
- 别名
- SEBOV GP (SEBOV GP 产品)
- 背景
- The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
- 分子量
- 33.8kDa
- UniProt
- Q7T9D9
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