Sonic Hedgehog Protein (SHH) (AA 24-197)
Our Local Distributor
北京 101111
Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN7319453)
抗原
See all Sonic Hedgehog (SHH) 蛋白蛋白类型
宿主
资源
纯度
-
-
产品特性
- AA 24-197
-
原理
- Recombinant Human Sonic Hedgehog/SHH Protein (C24II)
-
序列
- Cys24-Gly197(Cys24Ile-Ile)
-
产品特性
- Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197(Cys24Ile-Ile) is expressed.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Not validated for activity
-
-
Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (AA 1-198) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. CC Active
Sonic Hedgehog (SHH) (AA 25-198) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- Sonic Hedgehog (SHH)
-
别名
- SHH
-
背景
- HHG-1,SHH,Sonic Hedgehog Protein,Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45 kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the ""all signaling"" capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.
-
分子量
-
Calculated MW: 19.8 kDa
Observed MW: 20 kDa
-
基因ID
- 6469
-
UniProt
- Q15465
-
途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
-
