HCoV-OC43 N Protein (His tag)
HCoV-OC43 N
宿主: Human Coronavirus OC43 (HCoV-OC43)
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % as determined by SDS-PAGE.
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- 抗原 See all HCoV-OC43 N products
- HCoV-OC43 N (Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
- 蛋白类型
- Recombinant
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宿主
- Human Coronavirus OC43 (HCoV-OC43)
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HCoV-OC43 N protein is labelled with His tag.
- 原理
- Recombinant Human coronavirus (HCoV-OC43) Nucleoprotein / NP Protein (His Tag)
- 序列
- Met1-Ile448
- 产品特性
- A DNA sequence encoding the Human coronavirus (HCoV-OC43) Nucleoprotein (YP_009555245.1) (Met1-Ile448) was expressed with a polyhistide tag at the N-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE.
- 内毒素水平
- Please contact us for more information.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 40 mM PB, 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- HCoV-OC43 N (Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
- 别名
- Coronavirus OC-43 Nucleoprotein (HCoV-OC43 N 产品)
- 背景
- Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
- 分子量
- 50.43KDa.
- NCBI登录号
- YP_009555245
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